5 isoflavones, puerarin, daidzin, daidzein, 3′-hydroxy-puerarin and 3′-methoxy-puerarin had been isolated and purified concurrently from Pueraria lobata for the primary time by high efficiency counter-present chromatography (HPCCC) using a system consisting of hexane-ethyl acetate-n-butanol-ethanol-water (0.5 : 2 : 1 : 0.5 : 3.5, v/v/v/v/v). In whole, 155 mg of puerarin, 41 mg of daidzin, 12… Read more »
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