4. Xu L, Zheng N, He Q, Li R, Zhang K, Liang T. Puerarin, isolated from Pueraria lobata (Willd.), protects against hepatotoxicity by way of specific inhibition of the TGF-β1/Smad signaling pathway, thereby leading to anti-fibrotic impact.
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The entire phenolic content material of the P. lobata extract was measured primarily based on the method described previously with some minor modifications (17). The P. lobata extract was dissolved in methanol to a focus of 0.25 mg/ml. Elevated phenolic content and enhanced antioxidant exercise in fermented glutinous rice supplemented with fu brick tea. 24. Cherdshewasart W, Sutjit W. Correlation of antioxidant activity and major isoflavonoid contents of the phytoestrogen-wealthy Pueraria mirifica and Pueraria lobata tubers. Pervious study suggested that P. lobata extract was carefully associated to antioxidant, antiproliferative, and anti-inflammation results through cell models (15). However, few research paid attention to elucidate the connection and mechanism of the effect of P. lobata extract on the antioxidant actions and the composition of gut microbiota. 44. Picard C, Fioramonti J, Francois A, Robinson T, Neant F, Matuchansky C. Review article: bifidobacteria as probiotic brokers – physiological effects and clinical advantages. The well being benefits of P. lobata extract could possibly be higher understood in accordance with the direct comparability of microbial composition and construction by way of handled by P. lobata extract.
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The Student’s t-test was used to assess the mean difference between the P. lobata extract and the management teams. Ascorbic acid (vitamin C) was used as a optimistic control. Complete genome DNA was extracted from the fecal samples of the control and the P. lobata extract groups using a QIAamp DNA stool equipment, and the DNA concentration and purity were monitored on 1% agarose gels, and then diluted to 1 ng/μl for amplicon technology. In addition, there is a have to characterize a practical P. lobata extract and display its bioactive properties in vitro and in vivo.
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The P. lobata extract was separated utilizing an Agilent ZORBAX Eclipse Plus C18 (250 mm × 4.6 mm, 5.Zero μm particle size).