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Desk 3. Mutagenic and antimutagenic activity assay of Pueraria lobata and P. mirifica extracts based on non-metabolic activation in a rec assay utilizing Bacillus subtilis var. The Ames reversion assay was carried out on the sample options with and without the S9 mixture using the TA98 and TA100 indicator strains (22). The experiments had been carried out at a plant extract focus of 0.25-10 mg/plate. Therefore, given the growing publicity to plant phytoestrogens, in the current study we evaluated the P. mirifica and P. lobata extracts in in vitro and in vivo studies utilizing the Ames check for mutagenic and anti-mutagenic assays and the micronucleus check for genotoxicity assays to evaluate the genetic danger or safety of the two plant materials. For each the TA98 and TA100 reporter isolates, the presence of the S9 mixture initiated a higher frequency of revertant colonies in the destructive and optimistic controls as well as in the take a look at samples including puerarin, P. mirifica and P. lobata extracts.