To assess the estrogenic actions of synthetic estrogen, synthetic phytoestrogen, Pueraria lobata and three distinct cultivars of Pueraria mirifica, a phytoestrogen-rich herb, a vaginal cytology assay in ovariectomized rats had been used. Although each uterotropic and vaginal cytology assays can be used to assess the estrogenic activity of phytoestrogen-wealthy herb, nevertheless, using vaginal cytology assay has two advantages: (1) we don’t have to kill the animals and (2) we are able to observe up the restoration after the cessation of treatment. The incidence of vaginal cornification after remedy and the recovery after the cessation was dependent on dosages and cultivars of Pueraria mirifica. The increments of uterus weight in all rats agreed with the cornification of vaginal epithelium.
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The therapies of DW, genistein and Pueraria lobata didn’t influence the vaginal epithelium, however the injection of estradiol valerate induced a vaginal cornification from day-three of treatment to day-14 of post-treatment interval. Rats have been ovariectomized and treated with DW, estradiol valerate (1 mg/kg BW), genistein (0.25-2.5 mg/kg BW), Pueraria lobata and Pueraria mirifica (10-1,000 mg/kg BW) for 14 days.
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