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This analysis revealed numerous DEGs that were either uniquely expressed or up-regulated in the roots. Expressed unigenes in tissues depicted as boxplots. Statistical comparisons of the medians were made with the Kruskal-Wallis nonparametric test. KEGG annotation of Pueraria lobata unigenes. Representative chromatograms of the isoflavonoid analyzed in Pueraria lobata three tissues. On the xaxis is the retention time; on the y-axis are the intensity values. Distribution of number of unigenes having different expression levels in tissues. Number of unigenes that participated in the metabolites’ classification (A) and other secondary metabolites’ classification (B). Analysis of expression profiles in Pueraria lobata three tissues (roots, leaves, and stems). Our comprehensive transcriptome investigation substantially expands the genomic resources of P. lobata and provides valuable knowledge on both gene expression regulation and promising candidate genes that are involved in plant isoflavonoid pathways. Finally, quantitative analyses of isoflavonoid metabolites occurring in the three P. lobata tissue were done via high-performance liquid-chromatography and tandem mass spectrometry methodology (HPLC-MS/MS).

Pueraria Lobata Seeds

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Pueraria mirifica since 1991 and then later on Butea Superba. The scope of our study is biotechnology of the White, Red and Black Kwao Krua. The Thai vine herbal plant is grown in with the moderate-size trees ranking from timber wood to bamboo in the deciduous rain forest. Pueraria mirifica (White Kwao Krua) is Thailand’s endemic species found mainly in the northern and western of Thailand and has local name as “White Kwao Krua”. Our study has be done by traveling to many forests in many parts of Thailand to search and select for the right and best cultivar of these herbs by rely on botanical characteristic, chemical analysis, consumption history, toxicology test as well as laboratory test and clinical trial. The enlargement underground tuber accumulates “phyto-estrogens” (Hormone from Plant) comprising isoflavones (daidzin, daidzein, genistin, genistein and puerarin) and others such as miroestrol and its derivatives, beta-sitosterol, stigmasterol, coumestrol, puerarin, mirificoumestan, kwakhurin and mirificin.

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